(CNN) -- Soldiers from NATO's International Security Assistance Force fired on a vehicle after it failed to stop at a checkpoint in southern Afghanistan, killing four civilians, according to an ISAF statement.
The incident took place Saturday in the Sangin district of Helmand province, the statement said.
After directing the vehicle to stop, the soldiers fired warning shots, "but were eventually forced to fire at it when it refused to stop, fearing an insurgent attack," the ISAF statement said.
Three wounded civilians were treated for their injuries. Two other people in the vehicle were not hurt.
"ISAF deeply regrets this unnecessary incident caused by the reckless actions of the vehicle driver," the statement said, adding the incident will be investigated.
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