Some 54,000 people of a total 80,000 population were ordered to leave their homes at 8 a.m. (2 a.m. ET), said Francesca Cuomo, a press officer at Brindisi City Hall.
This is the first WWII bomb found in the city since 1945, and the evacuation is the largest undertaken in Italy since the end of the war, she added.
The British-made bomb -- which measures more than three feet in length and weighs 500 pounds -- was dropped in 1941.
It had lain untouched until it was found on November 2 by builders working to expand a local cinema.
The bomb was damaged during the operation, making it riskier than previous bomb disposals in Italy, Cuomo said.
People were allowed to return home at 1 p.m. (7 a.m. ET) Sunday after it was successfully removed.
A controlled explosion of the bomb will take place Monday in a sand quarry outside Brindisi, where it was taken on Sunday, said Lt. Col. Vincenzo Lipari of the Italian Armed Forces.
Defense Minister Lorenzo Guerini tweeted that the operation was "particularly complex" and congratulated those involved.